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themetagenomics provides functions to explore topics generated from 16S rRNA sequencing information on both the abundance and functional levels. It also provides an R implementation of PICRUSt and wraps Tax4fun, giving users a choice for their functional prediction strategy.

Relevant Software and Literature

Stephen Woloszynek, Joshua Chang Mell, Zhengqiao Zhao, Gideon Simpson, Michael P. O’Connor, and Gail Rosen. Exploring thematic structure and predicted functionality of 16S rRNA amplicon data. 2019. PLoS ONE 14(12); doi:

Margaret E. Roberts, Brandon M. Stewart, and Dustin Tingley (2017). stm: R Package for Structural Topic Models, 2016.

Morgan Langille et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. PICRUSt 1.1.1

Kathrin P. Aßhauer, Bernd Wemheuer, Rolf Daniel, and Peter Meinicke. Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data

Stan Development Team. 2016. RStan: the R interface to Stan. R package version 2.14.1., 2017.


# To install the release version of Themetagenomics:

# To install the developmental version via Github:
# install.packages('devtools')


For future feature requests or suggestions, request an issue: